KMID : 0363120190320020087
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Korean Journal of Pain 2019 Volume.32 No. 2 p.87 ~ p.96
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Effect of sec-O-glucosylhamaudol on mechanical allodynia in a rat model of postoperative pain
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Koh Gi-Ho
Song Hyun Kim Sang-Hun Yoon Myung-Ha Lim Kyung-Joon Oh Seon-Hee Jung Ki-Tae
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Abstract
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Background: This study was performed in order to examine the effect of intrathecal sec-O-glucosylhamaudol (SOG), an extract from the root of the Peucedanum japonicum Thunb., on incisional pain in a rat model.
Methods: The intrathecal catheter was inserted in male Sprague-Dawley rats (n = 55). The postoperative pain model was made and paw withdrawal thresholds (PWTs) were evaluated. Rats were randomly treated with a vehicle (70% dimethyl sulfoxide) and SOG (10 ¥ìg, 30 ¥ìg, 100 ¥ìg, and 300 ¥ìg) intrathecally, and PWT was observed for four hours. Dose-responsiveness and ED50 values were calculated. Naloxone was administered 10 min prior to treatment of SOG 300 ¥ìg in order to assess the involvement of SOG with an opioid receptor. The protein levels of the ¥ä-opioid receptor, ¥ê-opioid receptor, and ¥ì-opioid receptor (MOR) were analyzed by Western blotting of the spinal cord.
Results: Intrathecal SOG significantly increased PWT in a dose-dependent manner. Maximum effects were achieved at a dose of 300 ¥ìg at 60 min after SOG administration, and the maximal possible effect was 85.35% at that time. The medial effective dose of intrathecal SOG was 191.3 ¥ìg (95% confidence interval, 102.3?357.8). The antinociceptive effects of SOG (300 ¥ìg) were significantly reverted until 60 min by naloxone. The protein levels of MOR were decreased by administration of SOG.
Conclusions: Intrathecal SOG showed a significant antinociceptive effect on the postoperative pain model and reverted by naloxone. The expression of MOR were changed by SOG. The effects of SOG seem to involve the MOR.
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KEYWORD
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Analgesia, Blotting, western, Dimethyl sulfoxide, Hyperalgesia, Nociceptive pain, Pain, postoperative, Rats, Receptors, opioid, Spinal cord, 11-hydroxy-sec-O-glocosylhamaudol
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